28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR)

Short Description:

Product Code

HWTS-CC003

Product Approval

CE

The kit is used for the in vitro qualitative detection of the 28 types of human papillomavirus (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53 , 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) nucleic acids in human urine, female cervical swab and female vaginal swab samples, but these human papillomaviruses can not be completely typed. It can only provide auxiliary means for the diagnosis and treatment of HPV infection.


Product Detail

Product Tags

Product name

28 Types of HPV Nucleic Acid Detection Kit (Fluorescence PCR)

Epidemiology

Human Papillomavirus (HPV) belongs to the Papillomaviridae family of a small-molecule, non-enveloped, circular double-stranded DNA virus, with a genome length of about 8000 base pairs (bp). HPV infects humans through direct or indirect contact with contaminated items or sexual transmission. The virus is not only host-specific, but also tissue-specific, and can only infect human skin and mucosal epithelial cells, causing a variety of papillomas or warts in human skin and proliferative damage to reproductive tract epithelium.

Cervical cancer is one of the most common malignant tumors of the female reproductive tract. Studies have shown that HPV persistent infections and multiple infections are one of the main causes of cervical cancer. Currently, the recognized effective treatments are still lack for cervical cancer caused by HPV, so early discovery and prevention of cervical infection caused by HPV is the key to preventing cervical cancer. It is of great significance to establish a simple, specific and rapid etiology diagnostic test for the clinical diagnosis and treatment of cervical cancer.

Channel

S/N Channel Type
PCR-Mix1 FAM 16, 18, 31, 56
VIC(HEX) Internal Control
CY5 45, 51, 52, 53
ROX 33, 35, 58, 66
PCR-Mix2 FAM 6, 11, 54, 83
VIC(HEX) 26, 44, 61, 81
CY5 40, 42, 43, 82
ROX 39, 59, 68, 73

Technical Parameters

Storage ≤ -18℃
Shelf-life 12 months
Specimen Type Urine sample, Female cervical swab sample, Female vaginal swab sample
CV <5.0%
LoD 300 Copies/mL
Specificity  There is no cross reaction with other reproductive tract related pathogens and human genomic DNA.
Applicable Instruments Applied Biosystems 7500 Real-Time PCR System, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR Systems, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System.

Work flow: See the Kit IFU for details

Solution 1 (Automated nucleic acid extraction)

Work flow See the Kit IFU for details1

Solution 2: (Macro & Micro-Test Sample Release Reagent)

Work flow See the Kit IFU for details2

Reagents need but not provided

Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (which can be used with Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50).

Consumables need but not provided

1.5mL of DNase/RNase free tubes, DNase/RNase free tips, 8-tube strips for PCR, tabletop centrifuge, tabletop shaker mixer.


  • Previous:
  • Next:

  • Write your message here and send it to us